With the great amelioration of unmanned aerial vehicle (UAV) technology and the gradual opening of low-altitude airspace authorized by national policies, low-altitude and low-speed UAV increasingly intimidates the security of key areas. On the basis of the advantages and disadvantages of domestic and overseas defense and control systems (fixed and mobile types), a new type of UAV defense and control system, integrating the two subsystems through wireless network technology, is proposed to fully compensate for their respective weaknesses, and achieve several key technologies such as multi-objective cooperative attack, remote assisting attack, etc. Then, a pilot application of the new UAV defense and control system was carried out at Shapotou Airport, Zhongwei, Ningxia. In the process, strong capabilities of the multi-target cooperative attack and remote assisting attack performed by the new UAV defense and control system were fully substantiated. The construction of this new UAV defense and control system is an important exploration of anti-UAV systems, which inspires the further development of new anti-UAV strategies.